Application

Rabbit anti-ORC1 Antibody, Affinity Purified has been used in immunoblotting (1:2,000).

Biochem/physiol Actions

The origin recognition complex (ORC) plays a key role in DNA replication. ORC1 is degraded in the S phase and re-synthesized in the G1 phase of the cell cycle. ORC1 is responsible for cell proliferation, apoptosis, invasion, and migration. ORC1 regulates cyclin E1 (CCNE1) gene expression and is involved in genomic maintenance. Downregulation of ORC1 and cyclins cause Meier–Gorlin syndrome.

Disclaimer

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General description

Origin recognition complex 1 (ORC1) is the largest subunit of ORC. The subunits are widely expressed in the human lung, bones, kidney, and uterus. The ORC1 gene is mapped to human chromosome 1p32.3.

Immunogen

The epitope recognized by PLA0221 maps to a region between residue 811 and 861 of human origin recognition complex subunit 1 using the numbering given in entry NP_004144.2 (GeneID 4998).

Other Notes

ORC1 (origin recognition complex, subunit 1) is one of six subunits that make up the origin of recognition complex (ORC). The ORC serves as a scaffold for proteins involved in the initiation of DNA replication. Very recently, ORC1 has been found to perform a function independent of its role in DNA replication. In a screen using siRNAs for human ORC proteins to assess their roles in centrosome biology, it was found that ORC1 plays a key role in controlling centriole and centrosome copy number in human cells.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide